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VWR Gel documentation ®& western blot imaging01. VWR® BASIC 03. VWR® CHEMI ONLY 05. VWR® TRANSILLUMINATORS02. VWR® SMART5 04. VWR® IMAGER SYSTEMS VWR® GEL DOCUMENTATION & WESTERN BLOT IMAGINGChoose from the cost effective VWR® Basic system to the most advanced VWR® ImagerCHEMI Premium for fluorescence and chemiluminescent imaging. The range includes modelsadapted to many different applications so you can choose the best solution for your work
View the potential configurations below
VWR Basic SMART5 CHEMI only Imager2 Imager CHEMI Premium UV light transillumination √ √ √ √ Blue light transillumination √ √ √ √ R/G/B and IR epi √ illumination UV light epi illumination √ √ White light epi illumination √ √ √ √ Converters (UV to √ √ √ √ white or blue light) Colorimetric gels (e.g. √ √ √ √ Coomassie, silver stains) Colony plates √ √ √ √ Slot blots √ √ Chemiluminescence √ √ Bioluminescence √ √ Stain-free √ VWR® BASIC COST EFFECTIVE, QUALITY IMAGING This entry level system can be used with UV, blue or visible light transilluminators. These flexible lighting options make the VWR® Basic system suitable for imaging a wide range of samples
‒‒ High resolution digital camera for high quality images with precise band separation ‒‒ Full PC interface enables control of camera with real time imaging ‒‒ Programmable capture settings VWR® Basic Technical specifications Camera Resolution 18 MP Sensor/image depth 24 bit (colour) Lens Manual zoom, 3x optical Using the VWR Basic gel documentation Power Rechargeable battery (supplied) system you can produce images of Data type JPG gels stained with many fluorescent and Interface USB 2.0 Image storage Mass storage colorimetric dyes, including: Viewing area 20x20 cm ‒‒ Coomassie Blue Illumination Compatible with VWR stand-alone UV, blue or white light transilluminators ‒‒ Silver stain with max. 20x20 cm filter size Sold separately See page 7 ‒‒ Ethidium bromide Darkroom ‒‒ GelRed™ Hood Sits directly on transilluminator ‒‒ GelGreen™ Stand-alone and PC-operated (optional) PC sold separately ‒‒ EZ-VISION Image viewing area 20x20 cm Filter(s) ‒‒ SYBR® Gold/Green/Safe UV/blue filter Included ‒‒ SYPRO® Red/Ruby Software ‒‒ Fluorescein VWR® Image Capture Software Included VWR® Gel Documentation Software (for analysis) Included (1 license) As new dyes are released, we work to Dimensions optimise their use with the Basic system. HxDxW 37x21x28 cm Contact our technical support team if you need information on imaging dyes. Description Includes Cat. No
18 MP camera, hood, UV filter, 1 copy of the VWR Gel Documentation VWR® Basic Software.Transilluminator and converter not included 730-14702 VWR® Gel documentation & western blot imaging | vwr.com VWR® GEL DOCUMENTATION & WESTERN BLOT IMAGING VWR® SMART5 QUICK AND EASY STAND ALONE SYSTEM VWR® SMART5 is a new generation, low cost, integrated imaging system for DNA and protein analysis and gel documentation
‒‒ Compact workstation ‒‒ Complete with built-in processor, touch screen and SMART5 software ‒‒ Motor driven zoom lens ‒‒ 5 MP pixel camera ‒‒ White, UV and blue lighting options ‒‒ Complete networking connectivity (optional) SMART5 is the ultimate in compact gel documentation workstations. Using a choice of UV, blue or white lighting options you can capture and edit images of fluorescent ethidium bromide, SYBR® Safe or GelGreen™ DNA gels and protein gels stained with Coomassie Blue at the workstation
For an entry level system, the SMART5 comes with a ground breaking 5 million pixel CCD camera giving superb pixel resolution and unrivalled sensitivity. SMART5 offers a maximum viewing area of 20×24 cm which is very large for such a small, compact unit
Complete system includes 5 MP camera with motor driven zoom lens f1.2; VWR® SMART5 gel documentation system epi white light for sample positioning; UV032 filter; image capture and can produce images of gels stained with a GeneTools analysis software. Transilluminator and converter screens not wide range of fluorescent and colorimetric supplied (available separately)
dyes, including: ‒‒ Coomassie Blue ‒‒ Silver stain VWR® SMART5 Technical specifications ‒‒ Ethidium bromide Camera 5 MP, 12/16 bit CCD ‒‒ GelRed™ Display 7” (17,8 cm) touchscreen ‒‒ GelGreen™ ‒‒ EZ-Vision® Dynamic range 3,6/4,8 (extended) ‒‒ peqGREEN Filters UV032 ‒‒ SYBR® Gold/Green/Safe Image area (mm) 200×240 ‒‒ SYPRO® Red/Ruby Lens 8 - 48 mm f/1.2 ‒‒ Fluorescein Software GeneTools analysis ‒‒ Pro-Q® Diamond Weight 20 kg ‒‒ Deep PurpleTM W×D×H 310×450×750 mm As new dyes are released, we work to optimise their use with the VWR Gel Documentation systems. Contact our Description Includes Cat. No
technical support team if you need Complete system includes 5 MP camera with motor driven zoom lens information on imaging dyes. f1.2; epi white light for sample positioning; UV032 filter; image capture and GeneTools analysis software. Transilluminator and converter VWR® SMART5 screens not supplied. See page 7 730-0496 vwr.com | VWR® Gel documentation & western blot imaging 3 VWR® GEL DOCUMENTATION & WESTERN BLOT IMAGINGVWR CHEMI only Technical specifications VWR® CHEMI ONLYCamera CHEMILUMINESCENCE IMAGINGImage resolution 4 MPSensor/image depth 16 bit (65,536 grayscales) The new VWR® CHEMI only is dedicated toLens Fixed, f/0,95 chemiluminescence imaging. This system is builtQE @ 425 nm 0,73 for high performance and automation featuringCooling DT -57 °C a next generation high quantum efficiency CCDIllumination camera for even greater sensitivity. VWR® CHEMIEpi white light YesUse with external PC Optional only allows automatic capture of a quality imageDarkroom of any Western blot with a single ‘click’
Cabinet PC-operated (PC sold separately) Western blot imaging has never been as easyImage viewing area 11x8 cm as with the VWR® CHEMI only. The systemSoftwareVWR Image Capture Software automatically selects the right imaging YesVWR Gel Documentation Software Yes (2 licenses) conditions for any blot regardless of theDimensions chemiluminescent reagents being used. AllHxDxW 37,4x47x46 cm chemiluminescence applications can easily be handled by the VWR® CHEMI only. Produce superb images even from the faintest of signals
The compact instrument sits on any laboratory bench taking up very little space
VWR CHEMI only can produce images of ® The VWR® CHEMI only is ideal for small blots (up to 11x8 cm). The cooled 16 bit camera Western blots stained with chemiluminescence (ΔT -57 °C) with fixed lens (f/0,95) and data feedback amazes with a great quantum substrates, including: efficiency (73% @ 425 nm) and high resolution (4 MP)
‒‒ VisiGlo™ HRP chemiluminescent substrates ‒‒ ECL Western blotting substrates Description Includes Cat. No
4 MP image resolution, 12/ 16 bit, 2 copies of VWR® Gel Documenation VWR® CHEMI only Software, darkroom, 730-1471 Image Capture Software. 730-1471 VWR® IMAGER SYSTEMS VWR® Imager2 gel documentation systems FLUORESCENCE AND CHEMILUMINESCENCE IMAGING can produce images of gels stained with many fluorescent and colorimetric VWR Imager systems offer a wide range of different options depending on the dyes, including: configuration. Suitable for colorimetric, fluorescence, chemiluminescence and bioluminescence applications
‒‒ Coomassie Blue ‒‒ GelStar® ‒‒ Silver stain ‒‒ SYPRO® Red/ Choose from the following models: ‒‒ Ethidium Ruby/Orange bromide ‒‒ Fluorescein ‒‒ VWR® Imager2 for colorimetric and fluorescence applications only ‒‒ GelRed™ ‒‒ Rhodamine Red™ ‒‒ VWR® Imager CHEMI Premium for standard applications plus chemiluminescence ‒‒ GelGreen™ ‒‒ Texas Red® and bioluminescence ‒‒ EZ-VISION ‒‒ Pro-Q® Diamond ‒‒ peqGREEN ‒‒ Deep Purple™ Each system features a CCD camera, a motorised 7-position filter wheel and a motor- ‒‒ SYBR® Gold/ ‒‒ GFP driven zoom lens to allow for a wide range of imaging applications
Green/Safe The Imager CHEMI Premium comes with a cooled camera (ΔT -57 °C) that also enables use in chemiluminescence and bioluminescence applications
VWR Imager CHEMI Premium also offers ® imaging of: Choose from a wide range of lighting options for both transillumination and epi illumination including UV, blue and white light
‒‒ Western blots ‒‒ Chemiluminescence The VWR® Imager’s simple design allows for a wide range of configurations. Select ‒‒ Bioluminescence from two cameras, many different filters and lighting options. The darkroom is fully ‒‒ Stain-free gels microprocessor controlled, so that functions such as camera settings, lens control (with ‒‒ Alexa Fluor® dyes data feedback on VWR® Imager CHEMI Premium), filter selection and lighting can all ‒‒ CF™ dyes be set up via PC (not included). In addition, optional settings such as manual or auto ‒‒ Cy® dyes exposure time, neutral fielding and extended dynamic range are easily accessible for the user. All settings can be saved in user-defined configurations
We work with many more dyes than listed above, and as new dyes are released, we work to optimise their use with the VWR Imager range so please ask your VWR contact for updates
4 VWR® Gel documentation & western blot imaging | vwr.com VWR® GEL DOCUMENTATION & WESTERN BLOT IMAGING Technical specifications VWR® Imager2 VWR® Imager CHEMI Premium Camera Image resolution 3,8 MP 4 MP Sensor/image depth 12/ 16 bit (4,096 /65,536 grayscales) 16 bit (65,536 grayscales) Motor-driven zoom, f/1,2 with data Lens Motor-driven zoom, f/1,2 feedback QE @ 425 nm N/A 0,73 Cooling No DT -57 °C Illumination VWR UV and blue light transilluminators Sold separately Sold separately Epi white light Yes Yes Epi UV light modules Sold separately Sold separately Epi R/G/B and IR LED modules No Sold separately Visible light converter (UV to white light) Sold separately Sold separately Blue light converter screen (UV to blue light) Sold separately Sold separately Darkroom Cabinet PC-operated (PC sold separately) PC-operated (PC sold separately) Filter wheel Yes (7-position, motor-driven) Yes (7-position, motor-driven) Image viewing area 25,5x21 cm 30,5x22,7 cm Software VWR® Image Capture Software Yes Yes VWR® Gel Documentation Software (for analysis) Yes (2 licenses) Yes (2 licenses) Dimensions HxDxW 84x45x57 cm 84x45x57 cm Description Cat. No
VWR® Imager2 3,8 MP 12/ 16 bit camera, 7-position filter wheel, 2 copies of VWR® Gel Documentation Software, darkroom, VWR® Image Capture Software. Transilluminator and converter not supplied. See page 7 730-1458 VWR® Imager CHEMI Premium 4 MP image resolution, 12/ 16 bit, 7-position filter wheel, 2 copies of VWR® Gel Documentation Software, darkroom, LED gantry, VWR® Image Capture Software
Transilluminator and converter not supplied. See page 7 730-1469Cameras ‒‒ Blue light transilluminator for safe dyes such as GelRed™, GelGreen™, SYBR® dyes and EZ-Vision (20x16 cm illuminationBoth VWR® Imager systems are fitted with CCD cameras. The area)VWR® Imager2 is equipped with a 3,8 MP camera, enabling ‒‒ Visible light converter screen enabling white lightanalysis of e.g. colorimetric and fluorescent gels. The VWR® CHEMI transillumination for colorimetric applications such asPremium offers a 16-bit cooled (ΔT -57 °C) CCD camera with a Coomassie Blue and silver stainsresolution of 4 MP, allowing imaging of colorimetric, fluorescence, ‒‒ Blue light converter screen enabling blue light transilluminationchemiluminescence and bioluminescence applications. for 'Safe' dyes such as GelRed™, GelGreen™, SYBR® dyes and EZ- Vision. UV light epi illuminationLenses (254, 302 and 365 nm) ‒‒ White light epi illumination (standard for all VWR Imagers)All VWR® Imager systems record lens settings in order to meet GLP ‒‒ Epi illumination for R/G/B and IR fluorescence multiplex-imagingrequirements. They are equipped with fast f/1,2 zoom lenses and (only for VWR® Imager CHEMI Premium. Fitting LED modules candirectly controlled by the VWR® Image Capture Software. Both be added at any time in the future)VWR Imagers deliver a superb optical performance. ‒‒ Neutral fielding screensDarkroom FiltersThe units incorporate a fully light-tight darkroom best suited VWR Imager systems are fitted with an orange UV filter (572 – 625for chemiluminescent and fluorescent imaging (e.g. UV/blue nm) as standard. There are a number of additional filters that covertransillumination and UV, R/G/B and IR epi illumination). With a an extensive list of applications
wide opening door and a sliding transilluminator (optionally UV,blue or white light), VWR Imagers offer maximum convenience. ‒‒ Short pass filter - 516 – 600 nmThe electronic door locks automatically if exposures over 30 ‒‒ Long pass filter - 611 – 641 nmseconds are selected to prevent accidental interruption of imaging ‒‒ Far right red filter - 670 – 780 nmproceedings. VWR Imager units are built in a modular form to ‒‒ Filters for R/G/B multiplex imagingallow a wide choice of accessories to extend applications. The ‒‒ Filters for Q-DotsVWR® Imager2 is also fully upgradable for chemiluminescence For more information please contact your local VWR sales office
Computer (not supplied)Lighting The darkroom is fully microprocessor controlled. Camera settings,Range of lighting options lens control, filter selection and lighting are set-up via a PC. User-‒‒ UV transilluminators (254, 302 or 365 nm or a combination configurations can then be saved for future use. Please ask us for of any 2 wavelengths, either with 20x20 cm or 25x30 cm minimum computer requirements
illumination area) vwr.com | VWR® Gel documentation & western blot imaging 5 VWR® GEL DOCUMENTATION & WESTERN BLOT IMAGING VWR® IMAGE CAPTURE SOFTWARE An advanced image capture software package specifically developed to simply the process of capturing images. Every VWR Imager system includes VWR® Image Capture Software
This fully automatic package controls camera integration, exposure time, lens and capture options, as well as auto-focus configurations. A complete database of dyes and reagents at your fingertips. A comprehensive post-processing tool box allows the user to add text, lines, circles, shapes and arrows to the captured images. One button technology – a single button is all that is required to capture the perfect image every time. Lens control for operating the aperture, focus and zoom position for the motor- driven lens (with displayed feedback data)
‒‒ Full on-screen control of darkroom, camera and lens functions ‒‒ Auto switch-off function for UV lighting ‒‒ 'Live image’ feature in real time for easy focusing and positioning ‒‒ Automatic exposure control even for chemiluminescent imaging ‒‒ Series capture’ for a series of images over different time periods (e.g. for determination of ideal exposure times for chemiluminescent samples ‒‒ Saturation detection for quantitative applications ‒‒ Image annotations, enhancement and manipulation ‒‒ Image sharpening and smoothing ‒‒ Style control (lines, size, colour, fonts) ‒‒ Image cropping ‒‒ Save images in SGD private file format for true GLP ‒‒ Export images in various formats (TIF, BMP, JPEG) ‒‒ Direct link to the VWR® Gel Documentation Software for analysis VWR® GEL DOCUMENTATION SOFTWARE VWR® Gel Documentation Software is an advanced analysis software for use with any VWR Imager system. This highly automated software can rapidly analyse an image of the gel in a matter of seconds. Simplicity is the key feature of the VWR® Gel Documentation Software, results can be obtained in a click of a button and with minimal training
FEATURES OF THE VWR® GEL DOCUMENTATION SOFTWARE ID analysis ‒‒ Automatic counting/manual ‒‒ MW/BP analysis detection ‒‒ Automatic lane and peak detection ‒‒ Two colour counting ‒‒ Single or multiple gel analysis ‒‒ Automatic separator ‒‒ Automatic compensation for smiling ‒‒ Light/dark colony selection or distorted bands – no skewing ‒‒ Exclude regions function required ‒‒ Sensitivity selector ‒‒ MW/BP/Quantity calibrations ‒‒ RGB multiplex analysis ‒‒ Extensive reporting ‒‒ Spot analysis Extensive applications and dye ‒‒ Spot grid – automatic imaging ‒‒ Spot thresholding ‒‒ ®RGB multiplex analysis ‒‒ Quantitative and incidence analysis ‒‒ Quantitative dots (Q-dots) ‒‒ Multiple background subtraction ‒‒ SYBR® dyes methods ‒‒ Cy® dyes ‒‒ Alexa Fluor® dyes Colony counting ‒‒ SYPRO® dyes ‒‒ Advanced colony counting ‒‒ Pro-Q® dyes algorithm ‒‒ Plus many more6 VWR® Gel documentation & western blot imaging | vwr.com VWR® GEL DOCUMENTATION & WESTERN BLOT IMAGING VWR® TRANSILLUMINATORS All UV transilluminators slide out for easy access and offer homogeneous illumination for the best performance
Main features and choices ‒‒ Safety cut-off (when in darkroom) ‒‒ Slide out of darkroom for easy access and sample positioning ‒‒ Different single and dual wavelengths available ‒‒ Different filter sizes available 730-1461 Ordering information Transilluminator 20x20 cm, 302 nm TRANSILLUMINATORS FOR VWR® BASIC Transilluminator Blue slim is small and less expensive. 10x12cm sample size
Transilluminator Blue is larger and works best with the VWR® Imager2 and VWR® Chemi Premium darkrooms. Plus can be used with the VWR® Basic as this is bench top unit Description Cat. No
Transilluminator 20x20 cm, 302 nm 730-1464 Transilluminator 20x20 cm, 365 nm 730-1465 Transilluminator 20x20 cm, 254 nm 730-1466 Transilluminator Blue, 20x16 cm, 488 nm 730-1467 Visible light converter (UV to white light) 730-1395 VWR TRANSILLUMINATORS FOR VWR SMART5 Description Cat. No
Transilluminator 20x20 cm, 302 nm 730-1461 730-1389 Transilluminator 20x20 cm, 365 nm 730-1462 Transilluminator Blue, 20x16 cm, 470 nm Transilluminator 20x20 cm, 254 nm 730-1463 Transilluminator Blue slim, 10x12 cm, 470 nm 730-1468 Transilluminator 20×24 cm, 302 nm, with runners 730-0497 Visible light converter (UV to white light) 730-1395 Blue light converter (UV to blue light) 730-1493 TRANSILLUMINATORS FOR IMAGER2 AND CHEMI PREMIUM Description Cat. No. Description Cat. No
Transilluminator 20x20 cm, 302 nm 730-1482 Epi UV module 302 nm 730-1393 Transilluminator 25x30 cm, 302 nm 730-1483 Epi UV module 365 nm 730-1392 Transilluminator 20x20 cm, 365 nm 730-1472 Only for VWR® Imager CHEMI Premium Transilluminator 25x30 cm, 365 nm 730-1473 Epi-LED blue module 733-2369 Transilluminator 20x20 cm, 302/ 365 nm 730-1474 Epi-LED blue multiplexing module 733-2314 Transilluminator 25x30 cm, 302/ 365 nm 730-1475 Epi-LED red module 733-2371 Transilluminator 20x20 cm, 254 nm 730-1476 Epi-LED red multiplexing module 733-2372 Transilluminator 25x30 cm, 254 nm 730-1477 Epi-LED green module 733-2370 Transilluminator 20x20 cm, 254/ 365 nm 730-1478 Epi-LED green multiplexing module 733-2315 730-1390 Transilluminator 25x30 cm, 254/ 365 nm 730-1479 Epi-LED IR 740 multiplexing module 733-2316 Transilluminator Blue Slim 10x12 cm, 470 nm Filter for epi-LED module, 800 nm (range 809 Transilluminator 20x20 cm, 254/ 302 nm 730-1480 Transilluminator 25x30 cm, 254/ 302 nm 730-1481 - 876; LiCor IRDye800), Chemi systems only 730-1524 525 nm filter for use with blue Transilluminator Blue, 20x16 cm, 470 nm 730-1389 multiplexing LEDs 733-2305 Transilluminator Blue Slim, 10x12 cm, 470 nm 730-1390 Filter for Licor multiplexing 800 nm (809 - GX-Convert5 visible light converter (UV to 876 nm) 730-1524 white light) 730-1396 605 nm filter for use with green multiplexing Blue light converter 25x30cm 730-1494 LEDs 733-2480 Blue light converter 21x26cm 730-1493 705 nm filter for use with red multiplexing White light pad with brackets, 20x14 cm 730-1391 LEDs 733-2481 Epi UV module 254 nm 730-1394 PRINTER OPTIONS VWR® Smart5, VWR® Imager2, VWR® Imager CHEMI Premium, VWR® CHEMI only can all be used with the following digital thermal printer and paper
Description Cat. No
Thermal printer, digital 730-1260 730-1260 Thermal paper, matt 730-2892 Thermal printer, digital Thermal paper, glossy 733-2000 vwr.com | VWR® Gel documentation & western blot imaging 7 Setting science in motionto create a better world AUSTRIA GERMANY POLAND CHINA VWR International GmbH VWR International GmbH VWR International Sp. z o.o. VWR International China Co., Ltd
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VWR® GEL DOCUMENTATION & WESTERN BLOT IMAGING vwr.com VWR® Gel documentation & western blot imaging 5 Technical specifications VWR® Imager2 VWR® …
Specifically, western blot imaging systems allow for the visualization and study of target proteins within a mixture of proteins. Gel imaging is also commonly used in molecular biology, as it enables researchers to study nucleic acids using the same gel-based techniques used in western blotting immunoassays.
Our self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and helps your blots look their best. Details on blotting technology, available products, and methods, plus tips, techniques, and troubleshooting.
For Western blot analysis of BN gels, the protocol of Wittig et al. (2006) was adapted as follows. The gel was incubated in 300 mM Tris, 100 mM acetic acid, 1% SDS (pH 8.6) for 20 min with gentle shaking and then kept between glasses embedded with same solution for 1 h.
A western blot can also be used as a confirmatory test for Hepatitis B infection and HSV-2 (Herpes Type 2) infection. In veterinary medicine, a western blot is sometimes used to confirm FIV+ status in cats. Further applications of the western blot technique include its use by the World Anti-Doping Agency (WADA).