Vwr Gel Documentation Western Blot Imaging

Vwr gel documentation western blot imaging

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VWR Gel documentation
& western blot imaging
Choose from the cost effective VWR® Basic system to the most advanced VWR® Imager
CHEMI Premium for fluorescence and chemiluminescent imaging. The range includes models
adapted to many different applications so you can choose the best solution for your work

View the potential configurations below

VWR Basic SMART5 CHEMI only Imager2 Imager CHEMI Premium
UV light transillumination √ √ √ √
Blue light transillumination √ √ √ √
R/G/B and IR epi √
UV light epi illumination √ √
White light epi illumination √ √ √ √
Converters (UV to √ √ √ √
white or blue light)
Colorimetric gels (e.g. √ √ √ √
Coomassie, silver stains)
Colony plates √ √ √ √
Slot blots √ √
Chemiluminescence √ √
Bioluminescence √ √
Stain-free √
This entry level system can be used with UV, blue or visible light
transilluminators. These flexible lighting options make the VWR® Basic system
suitable for imaging a wide range of samples

‒‒ High resolution digital camera for high quality images with precise band
‒‒ Full PC interface enables control of camera with real time imaging
‒‒ Programmable capture settings
VWR® Basic Technical specifications
Resolution 18 MP
Sensor/image depth 24 bit (colour)
Lens Manual zoom, 3x optical
Using the VWR Basic gel documentation Power Rechargeable battery (supplied)
system you can produce images of Data type JPG
gels stained with many fluorescent and Interface USB 2.0
Image storage Mass storage
colorimetric dyes, including: Viewing area 20x20 cm
‒‒ Coomassie Blue Illumination
Compatible with VWR stand-alone UV, blue or white light transilluminators
‒‒ Silver stain with max. 20x20 cm filter size Sold separately See page 7
‒‒ Ethidium bromide Darkroom
‒‒ GelRed™ Hood Sits directly on transilluminator
‒‒ GelGreen™ Stand-alone and PC-operated (optional) PC sold separately
‒‒ EZ-VISION Image viewing area 20x20 cm
‒‒ SYBR® Gold/Green/Safe
UV/blue filter Included
‒‒ SYPRO® Red/Ruby Software
‒‒ Fluorescein VWR® Image Capture Software Included
VWR® Gel Documentation Software (for analysis) Included (1 license)
As new dyes are released, we work to Dimensions
optimise their use with the Basic system. HxDxW 37x21x28 cm
Contact our technical support team if you
need information on imaging dyes. Description Includes Cat. No

18 MP camera, hood, UV filter, 1 copy of the VWR Gel Documentation
VWR® Basic Software.Transilluminator and converter not included 730-1470
2 VWR® Gel documentation & western blot imaging | vwr.com
VWR® SMART5 is a new generation, low cost, integrated imaging system
for DNA and protein analysis and gel documentation

‒‒ Compact workstation
‒‒ Complete with built-in processor, touch screen and SMART5 software
‒‒ Motor driven zoom lens
‒‒ 5 MP pixel camera
‒‒ White, UV and blue lighting options
‒‒ Complete networking connectivity (optional)
SMART5 is the ultimate in compact gel documentation workstations. Using
a choice of UV, blue or white lighting options you can capture and edit
images of fluorescent ethidium bromide, SYBR® Safe or GelGreen™ DNA
gels and protein gels stained with Coomassie Blue at the workstation

For an entry level system, the SMART5 comes with a ground breaking 5
million pixel CCD camera giving superb pixel resolution and unrivalled
sensitivity. SMART5 offers a maximum viewing area of 20×24 cm which is
very large for such a small, compact unit

Complete system includes 5 MP camera with motor driven zoom lens f1.2;
VWR® SMART5 gel documentation system epi white light for sample positioning; UV032 filter; image capture and
can produce images of gels stained with a GeneTools analysis software. Transilluminator and converter screens not
wide range of fluorescent and colorimetric supplied (available separately)

dyes, including:
‒‒ Coomassie Blue
‒‒ Silver stain VWR® SMART5 Technical specifications
‒‒ Ethidium bromide Camera 5 MP, 12/16 bit CCD
‒‒ GelRed™
Display 7” (17,8 cm) touchscreen
‒‒ GelGreen™
‒‒ EZ-Vision® Dynamic range 3,6/4,8 (extended)
‒‒ peqGREEN Filters UV032
‒‒ SYBR® Gold/Green/Safe Image area (mm) 200×240
‒‒ SYPRO® Red/Ruby Lens 8 - 48 mm f/1.2
‒‒ Fluorescein
Software GeneTools analysis
‒‒ Pro-Q® Diamond
Weight 20 kg
‒‒ Deep PurpleTM
W×D×H 310×450×750 mm
As new dyes are released, we work to
optimise their use with the VWR Gel
Documentation systems. Contact our Description Includes Cat. No

technical support team if you need Complete system includes 5 MP camera with motor driven zoom lens
information on imaging dyes. f1.2; epi white light for sample positioning; UV032 filter; image capture
and GeneTools analysis software. Transilluminator and converter
VWR® SMART5 screens not supplied. See page 7 730-0496
vwr.com | VWR® Gel documentation & western blot imaging 3
VWR CHEMI only Technical specifications VWR® CHEMI ONLY
Image resolution 4 MP
Sensor/image depth 16 bit (65,536 grayscales) The new VWR® CHEMI only is dedicated to
Lens Fixed, f/0,95 chemiluminescence imaging. This system is built
QE @ 425 nm 0,73
for high performance and automation featuring
Cooling DT -57 °C
a next generation high quantum efficiency CCD
camera for even greater sensitivity. VWR® CHEMI
Epi white light Yes
Use with external PC Optional
only allows automatic capture of a quality image
of any Western blot with a single ‘click’

Cabinet PC-operated (PC sold separately)
Western blot imaging has never been as easy
Image viewing area 11x8 cm
as with the VWR® CHEMI only. The system
VWR Image Capture Software
automatically selects the right imaging
VWR Gel Documentation Software Yes (2 licenses)
conditions for any blot regardless of the
Dimensions chemiluminescent reagents being used. All
HxDxW 37,4x47x46 cm chemiluminescence applications can easily be
handled by the VWR® CHEMI only. Produce
superb images even from the faintest of signals

The compact instrument sits on any laboratory bench taking up very little space

VWR CHEMI only can produce images of
The VWR® CHEMI only is ideal for small blots (up to 11x8 cm). The cooled 16 bit camera
Western blots stained with chemiluminescence (ΔT -57 °C) with fixed lens (f/0,95) and data feedback amazes with a great quantum
substrates, including: efficiency (73% @ 425 nm) and high resolution (4 MP)

‒‒ VisiGlo™ HRP chemiluminescent substrates
‒‒ ECL Western blotting substrates
Description Includes Cat. No

4 MP image resolution, 12/ 16 bit, 2 copies of VWR® Gel Documenation
VWR® CHEMI only Software, darkroom, 730-1471 Image Capture Software. 730-1471
VWR® Imager2 gel documentation systems
can produce images of gels stained
with many fluorescent and colorimetric VWR Imager systems offer a wide range of different options depending on the
dyes, including: configuration. Suitable for colorimetric, fluorescence, chemiluminescence and
bioluminescence applications

‒‒ Coomassie Blue ‒‒ GelStar®
‒‒ Silver stain ‒‒ SYPRO® Red/
Choose from the following models:
‒‒ Ethidium Ruby/Orange
bromide ‒‒ Fluorescein ‒‒ VWR® Imager2 for colorimetric and fluorescence applications only
‒‒ GelRed™ ‒‒ Rhodamine Red™ ‒‒ VWR® Imager CHEMI Premium for standard applications plus chemiluminescence
‒‒ GelGreen™ ‒‒ Texas Red® and bioluminescence
‒‒ EZ-VISION ‒‒ Pro-Q® Diamond
‒‒ peqGREEN ‒‒ Deep Purple™ Each system features a CCD camera, a motorised 7-position filter wheel and a motor-
‒‒ SYBR® Gold/ ‒‒ GFP driven zoom lens to allow for a wide range of imaging applications

Green/Safe The Imager CHEMI Premium comes with a cooled camera (ΔT -57 °C) that also enables
use in chemiluminescence and bioluminescence applications

VWR Imager CHEMI Premium also offers
imaging of: Choose from a wide range of lighting options for both transillumination and epi
illumination including UV, blue and white light

‒‒ Western blots
‒‒ Chemiluminescence The VWR® Imager’s simple design allows for a wide range of configurations. Select
‒‒ Bioluminescence from two cameras, many different filters and lighting options. The darkroom is fully
‒‒ Stain-free gels microprocessor controlled, so that functions such as camera settings, lens control (with
‒‒ Alexa Fluor® dyes data feedback on VWR® Imager CHEMI Premium), filter selection and lighting can all
‒‒ CF™ dyes be set up via PC (not included). In addition, optional settings such as manual or auto
‒‒ Cy® dyes exposure time, neutral fielding and extended dynamic range are easily accessible for
the user. All settings can be saved in user-defined configurations

We work with many more dyes than listed
above, and as new dyes are released, we
work to optimise their use with the VWR
Imager range so please ask your VWR
contact for updates

4 VWR® Gel documentation & western blot imaging | vwr.com
Technical specifications VWR® Imager2 VWR® Imager CHEMI Premium
Image resolution 3,8 MP 4 MP
Sensor/image depth 12/ 16 bit (4,096 /65,536 grayscales) 16 bit (65,536 grayscales)
Motor-driven zoom, f/1,2 with data
Lens Motor-driven zoom, f/1,2 feedback
QE @ 425 nm N/A 0,73
Cooling No DT -57 °C
VWR UV and blue light transilluminators Sold separately Sold separately
Epi white light Yes Yes
Epi UV light modules Sold separately Sold separately
Epi R/G/B and IR LED modules No Sold separately
Visible light converter (UV to white light) Sold separately Sold separately
Blue light converter screen (UV to blue
light) Sold separately Sold separately
Cabinet PC-operated (PC sold separately) PC-operated (PC sold separately)
Filter wheel Yes (7-position, motor-driven) Yes (7-position, motor-driven)
Image viewing area 25,5x21 cm 30,5x22,7 cm
VWR® Image Capture Software Yes Yes
VWR® Gel Documentation Software (for
analysis) Yes (2 licenses) Yes (2 licenses)
HxDxW 84x45x57 cm 84x45x57 cm
Description Cat. No

VWR® Imager2
3,8 MP 12/ 16 bit camera, 7-position filter wheel, 2 copies of VWR® Gel Documentation
Software, darkroom, VWR® Image Capture Software. Transilluminator and converter
not supplied. See page 7 730-1458
VWR® Imager CHEMI Premium
4 MP image resolution, 12/ 16 bit, 7-position filter wheel, 2 copies of VWR® Gel
Documentation Software, darkroom, LED gantry, VWR® Image Capture Software

Transilluminator and converter not supplied. See page 7 730-1469
Cameras ‒‒ Blue light transilluminator for safe dyes such as GelRed™,
GelGreen™, SYBR® dyes and EZ-Vision (20x16 cm illumination
Both VWR® Imager systems are fitted with CCD cameras. The area)
VWR® Imager2 is equipped with a 3,8 MP camera, enabling ‒‒ Visible light converter screen enabling white light
analysis of e.g. colorimetric and fluorescent gels. The VWR® CHEMI transillumination for colorimetric applications such as
Premium offers a 16-bit cooled (ΔT -57 °C) CCD camera with a Coomassie Blue and silver stains
resolution of 4 MP, allowing imaging of colorimetric, fluorescence, ‒‒ Blue light converter screen enabling blue light transillumination
chemiluminescence and bioluminescence applications. for 'Safe' dyes such as GelRed™, GelGreen™, SYBR® dyes and EZ-
Vision. UV light epi illumination
Lenses (254, 302 and 365 nm)
‒‒ White light epi illumination (standard for all VWR Imagers)
All VWR® Imager systems record lens settings in order to meet GLP ‒‒ Epi illumination for R/G/B and IR fluorescence multiplex-imaging
requirements. They are equipped with fast f/1,2 zoom lenses and (only for VWR® Imager CHEMI Premium. Fitting LED modules can
directly controlled by the VWR® Image Capture Software. Both be added at any time in the future)
VWR Imagers deliver a superb optical performance. ‒‒ Neutral fielding screens
Darkroom Filters
The units incorporate a fully light-tight darkroom best suited VWR Imager systems are fitted with an orange UV filter (572 – 625
for chemiluminescent and fluorescent imaging (e.g. UV/blue nm) as standard. There are a number of additional filters that cover
transillumination and UV, R/G/B and IR epi illumination). With a an extensive list of applications

wide opening door and a sliding transilluminator (optionally UV,
blue or white light), VWR Imagers offer maximum convenience. ‒‒ Short pass filter - 516 – 600 nm
The electronic door locks automatically if exposures over 30 ‒‒ Long pass filter - 611 – 641 nm
seconds are selected to prevent accidental interruption of imaging ‒‒ Far right red filter - 670 – 780 nm
proceedings. VWR Imager units are built in a modular form to ‒‒ Filters for R/G/B multiplex imaging
allow a wide choice of accessories to extend applications. The ‒‒ Filters for Q-Dots
VWR® Imager2 is also fully upgradable for chemiluminescence
For more information please contact your local VWR sales office

Computer (not supplied)
The darkroom is fully microprocessor controlled. Camera settings,
Range of lighting options
lens control, filter selection and lighting are set-up via a PC. User-
‒‒ UV transilluminators (254, 302 or 365 nm or a combination configurations can then be saved for future use. Please ask us for
of any 2 wavelengths, either with 20x20 cm or 25x30 cm minimum computer requirements

illumination area)
vwr.com | VWR® Gel documentation & western blot imaging 5
An advanced image capture software package specifically developed to simply
the process of capturing images. Every VWR Imager system includes VWR® Image
Capture Software

This fully automatic package controls camera integration, exposure time, lens and
capture options, as well as auto-focus configurations. A complete database of dyes
and reagents at your fingertips. A comprehensive post-processing tool box allows the
user to add text, lines, circles, shapes and arrows to the captured images. One button
technology – a single button is all that is required to capture the perfect image every
time. Lens control for operating the aperture, focus and zoom position for the motor-
driven lens (with displayed feedback data)

‒‒ Full on-screen control of darkroom, camera and lens functions
‒‒ Auto switch-off function for UV lighting
‒‒ 'Live image’ feature in real time for easy focusing and positioning
‒‒ Automatic exposure control even for chemiluminescent imaging
‒‒ Series capture’ for a series of images over different time periods (e.g. for
determination of ideal exposure times for chemiluminescent samples
‒‒ Saturation detection for quantitative applications
‒‒ Image annotations, enhancement and manipulation
‒‒ Image sharpening and smoothing
‒‒ Style control (lines, size, colour, fonts)
‒‒ Image cropping
‒‒ Save images in SGD private file format
for true GLP
‒‒ Export images in various formats
‒‒ Direct link to the VWR® Gel Documentation Software for analysis
VWR® Gel Documentation Software is an advanced analysis software for use with any
VWR Imager system. This highly automated software can rapidly analyse an image of the
gel in a matter of seconds. Simplicity is the key feature of the VWR® Gel Documentation
Software, results can be obtained in a click of a button and with minimal training

ID analysis ‒‒ Automatic counting/manual
‒‒ MW/BP analysis detection
‒‒ Automatic lane and peak detection ‒‒ Two colour counting
‒‒ Single or multiple gel analysis ‒‒ Automatic separator
‒‒ Automatic compensation for smiling ‒‒ Light/dark colony selection
or distorted bands – no skewing ‒‒ Exclude regions function
required ‒‒ Sensitivity selector
‒‒ MW/BP/Quantity calibrations ‒‒ RGB multiplex analysis
‒‒ Extensive reporting
‒‒ Spot analysis Extensive applications and dye
‒‒ Spot grid – automatic imaging
‒‒ Spot thresholding ‒‒ ®RGB multiplex analysis
‒‒ Quantitative and incidence analysis ‒‒ Quantitative dots (Q-dots)
‒‒ Multiple background subtraction ‒‒ SYBR® dyes
methods ‒‒ Cy® dyes
‒‒ Alexa Fluor® dyes
Colony counting ‒‒ SYPRO® dyes
‒‒ Advanced colony counting ‒‒ Pro-Q® dyes
algorithm ‒‒ Plus many more
6 VWR® Gel documentation & western blot imaging | vwr.com
All UV transilluminators slide out for easy access and offer
homogeneous illumination for the best performance

Main features and choices
‒‒ Safety cut-off (when in darkroom)
‒‒ Slide out of darkroom for easy access and sample positioning
‒‒ Different single and dual wavelengths available
‒‒ Different filter sizes available
Ordering information
Transilluminator 20x20 cm, 302 nm
Transilluminator Blue slim is small and less expensive. 10x12cm sample size

Transilluminator Blue is larger and works best with the VWR® Imager2 and VWR® Chemi
Premium darkrooms. Plus can be used with the VWR® Basic as this is bench top unit
Description Cat. No

Transilluminator 20x20 cm, 302 nm 730-1464
Transilluminator 20x20 cm, 365 nm 730-1465
Transilluminator 20x20 cm, 254 nm 730-1466
Transilluminator Blue, 20x16 cm, 488 nm 730-1467
Visible light converter (UV to white light) 730-1395
Description Cat. No

Transilluminator 20x20 cm, 302 nm 730-1461
730-1389 Transilluminator 20x20 cm, 365 nm 730-1462
Transilluminator Blue, 20x16 cm, 470 nm
Transilluminator 20x20 cm, 254 nm 730-1463
Transilluminator Blue slim, 10x12 cm, 470 nm 730-1468
Transilluminator 20×24 cm, 302 nm, with runners 730-0497
Visible light converter (UV to white light) 730-1395
Blue light converter (UV to blue light) 730-1493
Description Cat. No. Description Cat. No

Transilluminator 20x20 cm, 302 nm 730-1482 Epi UV module 302 nm 730-1393
Transilluminator 25x30 cm, 302 nm 730-1483 Epi UV module 365 nm 730-1392
Transilluminator 20x20 cm, 365 nm 730-1472 Only for VWR® Imager CHEMI Premium
Transilluminator 25x30 cm, 365 nm 730-1473 Epi-LED blue module 733-2369
Transilluminator 20x20 cm, 302/ 365 nm 730-1474 Epi-LED blue multiplexing module 733-2314
Transilluminator 25x30 cm, 302/ 365 nm 730-1475 Epi-LED red module 733-2371
Transilluminator 20x20 cm, 254 nm 730-1476 Epi-LED red multiplexing module 733-2372
Transilluminator 25x30 cm, 254 nm 730-1477 Epi-LED green module 733-2370
Transilluminator 20x20 cm, 254/ 365 nm 730-1478 Epi-LED green multiplexing module 733-2315
730-1390 Transilluminator 25x30 cm, 254/ 365 nm 730-1479 Epi-LED IR 740 multiplexing module 733-2316
Transilluminator Blue Slim 10x12 cm, 470 nm Filter for epi-LED module, 800 nm (range 809
Transilluminator 20x20 cm, 254/ 302 nm 730-1480
Transilluminator 25x30 cm, 254/ 302 nm 730-1481 - 876; LiCor IRDye800), Chemi systems only 730-1524
525 nm filter for use with blue
Transilluminator Blue, 20x16 cm, 470 nm 730-1389
multiplexing LEDs 733-2305
Transilluminator Blue Slim, 10x12 cm, 470 nm 730-1390
Filter for Licor multiplexing 800 nm (809 -
GX-Convert5 visible light converter (UV to
876 nm) 730-1524
white light) 730-1396
605 nm filter for use with green multiplexing
Blue light converter 25x30cm 730-1494 LEDs 733-2480
Blue light converter 21x26cm 730-1493 705 nm filter for use with red multiplexing
White light pad with brackets, 20x14 cm 730-1391 LEDs 733-2481
Epi UV module 254 nm 730-1394
VWR® Smart5, VWR® Imager2, VWR® Imager CHEMI Premium, VWR® CHEMI
only can all be used with the following digital thermal printer and paper

Description Cat. No

Thermal printer, digital 730-1260
730-1260 Thermal paper, matt 730-2892
Thermal printer, digital Thermal paper, glossy 733-2000
vwr.com | VWR® Gel documentation & western blot imaging 7
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Frequently Asked Questions

What is the difference between western blot and gel imaging?

Specifically, western blot imaging systems allow for the visualization and study of target proteins within a mixture of proteins. Gel imaging is also commonly used in molecular biology, as it enables researchers to study nucleic acids using the same gel-based techniques used in western blotting immunoassays.

What is the western blotting troubleshooting guide?

Our self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and helps your blots look their best. Details on blotting technology, available products, and methods, plus tips, techniques, and troubleshooting.

What is the protocol for western blot analysis of bn gels?

For Western blot analysis of BN gels, the protocol of Wittig et al. (2006) was adapted as follows. The gel was incubated in 300 mM Tris, 100 mM acetic acid, 1% SDS (pH 8.6) for 20 min with gentle shaking and then kept between glasses embedded with same solution for 1 h.

What is a western blot used for in veterinary medicine?

A western blot can also be used as a confirmatory test for Hepatitis B infection and HSV-2 (Herpes Type 2) infection. In veterinary medicine, a western blot is sometimes used to confirm FIV+ status in cats. Further applications of the western blot technique include its use by the World Anti-Doping Agency (WADA).